Part:BBa_K2259056:Design

SynORI constitutive plasmid copy number device for group A-NC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Mutations for group selection had to be carefully tailored in order not to change secondary structure of RNA I.

Source

This part is a modified version of wildtype ColE1 RNA I taken from addgene.